Weizmann Group
Synthetic nucleic acids and photothermal nanomaterials design group
Real-time detection of telomerase activity using the exponential isothermal amplification of telomere repeat assay
As crucial pieces in the puzzle of cancer and human aging, telomeres and telomerase are indispensable in modern biology. Here we describe a novel exponential isothermal amplification of telomere repeat (EXPIATR) assay - a sensitive, simple, and reliable in vitro method for measuring telomerase activity in cell extracts. Through a strategically designed path of nucleic acid isothermal amplifications, EXPIATR abandons the expensive thermal cycling protocol and achieves ultrafast detection: telomerase activity equivalent to a single HeLa cancer cell can be detected in ∼25 min.
| Publication language | English |
| Pages | 1661-1664 |
| Journal | Journal of the American Chemical Society |
| Volume | 135 |
| Issue number | 5 |
| Publication status | Published - 06.02.2013 |
ASJC Scopus subject areas
Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry
