Weizmann Group
Synthetic nucleic acids and photothermal nanomaterials design group
Autonomous fueled mechanical replication of nucleic acid templates for the amplified optical detection of DNA
(Figure Presented) Fuel for a DNA machine: A Fokl enzyme/DNA scission machine is triggered by the hybridization of its DNA hairpin structure with a target nucleic acid. The machine is fueled by a secondary fluorophore (F)- and quencher (Q)-functionalized nucleic acid 2. Scission of 3 leads to replication of the machinery cutter 1 and yields a fluorescent product 6, which provides an optical output for the machine (see scheme).
| Publication language | English |
| Pages | 2238-2242 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 45 |
| Issue number | 14 |
| Publication status | Published - 27.03.2006 |
Keywords
DNA
Enzymes
Fluorescence
Self-replication
ASJC Scopus subject areas
Catalysis
General Chemistry
