The Environmental Biotechnology Lab

The discovery of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated protein 9 (Cas9) system has emerged as a prominent system for genome engineering in diverse organisms. Biotechnological and industrial production of foods, beverages, chemicals, proteins and fuels is dependent on effective production systems, commonly utilizing the genetically modified organisms. CRISPR-Cas9 approach has been used for genome editing of wide range of fungi and yeast for industrial applications. It has enabled significant advances in mycology research by hastening the growth of study models for those aiming to discover and validate novel compounds. This chapter aims to bring a comprehensive detail on application of CRISPR-Cas9 mediated genome editing in different fungi toward industrial applications.