Alberto Bilenca

We propose and analyze the use of fluorescence phase-shifting interferometric (PSI) imaging for single particle tracking along 1 μm-depth (z) trajectories. While spatial PSI using a single camera attains a maximal twofold improvement in the z-localization precision compared to temporal PSI for <0.1 μm²/s diffusion, temporal PSI shows a fourfold larger field-of-view.