Maya Bar

Senior Academic

Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato

Evyatar Steiner, Alon Israeli, Rupali Gupta, Ido Shwartz, Ido Nir, Meirav Leibman-Markus, Lior Tal, Mika Farber, Ziva Amsalem, Naomi Ori, Bruno Müller, Maya Bar

Background: Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool. The phytohormone cytokinin (CK) has crucial roles in the regulation of plant growth and development. Results: Here we describe a version of the CK sensor Two Component signaling Sensor (TCS), referred to as TCSv2. TCSv2 has a different arrangement of binding motifs when compared to previous TCS versions, resulting in increased sensitivity in some examined tissues. Here, we examine the CK responsiveness and distribution pattern of TCSv2 in arabidopsis and tomato. Conclusions: The increased sensitivity and reported expression pattern of TCSv2 make it an ideal TCS version to study CK response in particular hosts, such as tomato, and particular tissues, such as leaves and flowers.

Publication language English
Volume 16
Issue number 1
Publication status Published - 01.12.2020

Keywords

Arabidopsis
Cytokinin
Imaging
TCSv2
Tomato
Two-component sensor (TCS)

ASJC Scopus subject areas

Biotechnology
Genetics
Plant Science
Access to Document
10.1186/s13007-020-00694-2
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Link to publication in Scopus