ELECTRO-OPTICS  LABORATORY

Department of Electrical and Computer Engineering

Quantitative phase-contrast by using a modified phase retrieval algorithm

Nathaniel Hai, Joseph Rosen

Spatial filtering-based quantitative phase imaging of transparent objects is demonstrated. By combining a standard phase-contrast microscopy technique with an optical system-mimicking Gerchberg-Saxton phase retrieval algorithm, we successfully transformed a qualitative phase map into an accurate quantitative phase distribution of the examined object. Feeding a phase-induced intensity image into the modified phase retrieval algorithm is found to facilitate the convergence and ambiguities issues associated with the standard algorithms. Accurate phase recovery for two different kinds of objects supports the use of the suggested method as a single-shot quantitative phase-contrast microscope.

Publication language English
Publication status Published - 01.01.2021
116530S

Keywords

Label-free phase imaging
Phase pinhole
Phase retrieval algorithm
Quantitative phase imaging
Quantitative phase-contrast

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging
Access to Document
10.1117/12.2576495
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Link to publication in Scopus