ELECTRO-OPTICS  LABORATORY

Department of Electrical and Computer Engineering

Theoretical and experimental demonstration of resolution beyond the Rayleigh limit by FINCH fluorescence microscopic imaging

Joseph Rosen, Nisan Siegel, Gary Brooker

Fresnel Incoherent Correlation Holography (FINCH) enables holograms to be recorded from incoherent light with just a digital camera and spatial light modulator. We previously described its application to general three dimensional incoherent imaging and specifically to fluorescence microscopy, wherein one complex hologram contains the three dimensional information in the field of view, obviating the need for scanning or serial sectioning. We have now further analyzed FINCH in view of linear system theory and in comparison to conventional coherent and incoherent two dimensional imaging systems. We demonstrate, theoretically and experimentally, improved resolution by FINCH, when compared to conventional imaging.

Publication language English
Pages 26249-26268
Journal Optics Express
Volume 19
Issue number 27
Publication status Published - 19.12.2011

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Access to Document
10.1364/OE.19.026249
Other files and links
Link to publication in Scopus