
ELECTRO-OPTICS LABORATORY
Parallel-mode scanning optical sectioning using digital Fresnel holography with three-wave interference phase-shifting
The Fresnel incoherent correlation holography (FINCH) method is applicable to various techniques of imaging, including fluorescence microscopy. Recently, a FINCH configuration capable of optical sectioning, using a scanning phase pinhole, has been suggested [Optica 1, 70 (2014)]. This capability is highly important in situations that demand the suppression of out-of-focus information from the hologram reconstruction of a specific plane of interest, such as the imaging of thick samples in biology. In this study, parallel-mode scanning using multiple phase pinholes is suggested as a means to shorten the acquisition time in an optical sectioning FINCH configuration. The parallel-mode scanning is enabled through a phase-shifting procedure that extracts the mixed term of two out of three interfering beams.
| Publication language | English |
| Pages | 2200-2214 |
| Journal | Optics Express |
| Volume | 24 |
| Issue number | 3 |
| Publication status | Published - 08.02.2016 |