ELECTRO-OPTICS  LABORATORY

Department of Electrical and Computer Engineering

Parallel-mode scanning optical sectioning using digital Fresnel holography with three-wave interference phase-shifting

Roy Kelner, Joseph Rosen

The Fresnel incoherent correlation holography (FINCH) method is applicable to various techniques of imaging, including fluorescence microscopy. Recently, a FINCH configuration capable of optical sectioning, using a scanning phase pinhole, has been suggested [Optica 1, 70 (2014)]. This capability is highly important in situations that demand the suppression of out-of-focus information from the hologram reconstruction of a specific plane of interest, such as the imaging of thick samples in biology. In this study, parallel-mode scanning using multiple phase pinholes is suggested as a means to shorten the acquisition time in an optical sectioning FINCH configuration. The parallel-mode scanning is enabled through a phase-shifting procedure that extracts the mixed term of two out of three interfering beams.

Publication language English
Pages 2200-2214
Journal Optics Express
Volume 24
Issue number 3
Publication status Published - 08.02.2016

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Access to Document
10.1364/OE.24.002200
Other files and links
Link to publication in Scopus