Ophthalmic Extracellular Vesicles Research Lab

Aim: To evaluate changes in the reducing power of aqueous humor (AH) with cyclic voltammetry (CV) and HPLC-EC. Methods: NZW Rabbits exhibiting a sporadic mutation causing bilaterally buphthalmus eyes were set for intra ocular pressure (IOP) and eye size measurements. AH was obtained under anesthesia, from congenital glaucomatic rabbits (CGR, n=6) and age-matched controls (CON; n=6). The AH samples were analysed by CV and HPLC-EC. Results: CGR IOP was found to be significantly higher than in CON (33.5±1.1 and 14.2±1.0 mmHg, respectively), eye size was 18.25 and 13.9 cm, respectively. CV analysis revealed two anodic currents representing two groups of low molecular weight antioxidant (LMWA). The two anodic potentials were equal for the two tested groups, indicating the same components of LMWA. The first anodic current of CGR was only 30% of the CON rabbits (2.11 vs 7.17μA/mg protein, t-test: P<0.05). As the main hydrophilic components of the first anodic current are known to be uric acid (UA) and ascorbic acid (AA), they were analysed for exact content by HPLC-EC. UA and AA levels were significantly lower in the CGR group (UA: 17.1±3.2 and 189.1±75.70μM/mg, AA: 1.1±0.3 and 4.8±2.0μM/mg protein respectively). Conclusions: Changes in the reducing power, as indicated by CV analysis, of CGR AH, is probably a result of chronic oxidative stress caused by the pathology. The differences in the first anodic wave are mainly due to a fall in the concentration of UA and AA.