We assembled giant vesicles (GVs) composed of different phospholipids embedded in the chromatic polydiacetylene matrix. Due to the micrometer size of these vesicles (10-20 µm), experimentation can be carried out using conventional microscopy, such as optical and fluorescence microscopies. Using the lipid/PDA GVs, we have studied size and morphology changes induced by varied membrane active compounds and microorganisms.