Bio-mimetic lipid/PDA vesicles

A schematic description of a chromatic vesicle composed of phospholipids (black) and polydiacetylene (PDA) (blue). These particles form bio-mimetic bilayers constituting a useful platform for studying membrane processes, such as peptide-membrane interactions, pore formation, drug permeation through lipid barriers, and others.


Relevant Publications
  1. Array-based disease diagnostics using lipid/polydiacetylene vesicles encapsulated in a sol-gel matrix, S. Kolusheva, R. Yossef, A. Kugel, M. Katz, R. Volinsky, M. Welt, U. Hadad, V. Drory, M. Kliger, E. Rubin, A. Porgador, R. Jelinek, Analytical Chemistry, 2012, 84, 5925–5931.
    [Full Paper]

  2. Biomolecular sensing with colourimetric vesicles, Raz Jelinek, Sofiya Kolusheva, Topics in Current Chemistry, 2007, vol. 277, (Schrader T., Ed.)
    [Full Paper]

  3. Rapid colorimetric screening of drug interaction and penetration through lipid barriers, Marina Katz, Izek Ben Shlush, Sofiya Kolusheva, Raz Jelinek, Pharmaceutical Research, 2006, 23(3), 580-588.
    [Full Paper]

  4. Membrane interactions of host defense peptides studied in model systems, R. Jelinek, S. Kolusheva, Curr. Prot. Pep. Sci., 2005, 6, 103-114.
    [Full Paper]

  5. Membrane binding and permeation by indolicidin analogs studied by a biomimetic lipid/polydiacetylene vesicle assay, R. Halevy, A. Rozek, S. Kolusheva, R. E. Hancock, R. Jelinek, Peptides., 2003, 24, 1753-1761.
    [Full Paper]

  6. Detection and analysis of membrane interactions by a biomimetic colorimetric lipid/polydiacetylene assay, S. Rozner, S. Kolusheva, Z. Cohen, W. Dowhan, J. Eichler, R. Jelinek, Analytical Biochemistry, 2003, 319, 96-104.
    [Full Paper]

  7. Bio-mimetic lipid/polymer colorimetric membranes: Molecular and cooperative properties, S. Kolusheva, E. Wachtel, R. Jelinek, J. Lipid Research, 2003, 44, 65-71.
    [Abstract]

  8. A colorimetric assay for rapid screening of anti-microbial peptides, S. Kolusheva, L. Boyer, R. Jelinek, Nature Biotechnology, 2000, 18, 225-227.
    [Abstract]

  9. Structure-Activity Determinants in Paneth Cell ±-Defensins: Loss-of-function in Mouse Cryptdin-4 by Charge-reversal at Arginine Residue Positions, H. Tanabe, X. Qu, J. Cummings, S. Kolusheva, C. S. Weeks, K. B. Walsh, R. Jelinek, T. K. Vanderlick, M. E. Selsted, and A. J. Ouellette, J. Biol. Chem., 2004, 279, 11976-11983.
    [Full Paper]

  10. Membrane binding and permeation by indolicidin analogs studied by a biomimetic lipid/polydiacetylene vesicle assay, R. Halevy, A. Rozek, S. Kolusheva, R.E. Hancock, R. Jelinek, Peptides., 2003, 24, 1753-1761.
    [Full Paper]

  11. Quantitative interactions between Cryptdin-4 amino terminal variants and membranes, D.P. Satchell, T. Sheynis, J. Cummins, T.K. Vanderlick, R. Jelinek, A. Ouellette, Peptides., 2003, 24, 1795-1805.
  12. Bilayer localization of membrane-active peptides studied in bio-mimetic vesicles by visible and fluorescence spectroscopies, T. Sheynis, J. Sykora, A. Benda, S. Kolusheva, M. Hof, R. Jelinek, Eur. J. Biochem., 2003, 270, 4478-4487.
    [Full Paper]

  13. The human islet amyloid polypeptide forms transient membrane-active protofilaments, Y. Porat, S. Kolusheva, R. Jelinek, E. Gazit, Biochemistry, 2003, 42, 10971-10977.
    [Abstract][Full Paper]

  14. Lipid binding and membrane penetration of polymyxin B nona-peptide derivatives: significance of LPS binding and effects of a hydrophbic side residue, M. Katz, H. Tsubery, M. Fridkin, S. Kolusheva, A. Shames, R. Jelinek, Biochem. J., 2003, 375, 405-413.
    [Abstract][Full Paper]

  15. Interactions of Mouse Paneth Cell a-Defensins and a-Defensin Precursors with Membranes: Prosegment Inhibition of Peptide Association with Biomimetic Membranes D.P. Satchell, T. Sheynis, Y. Shirafuji, S. Kolusheva, A. Ouellette, R. Jelinek, J. Biol. Chem., 2003, 278(16), 13838-13847.
    [Abstract][Full Paper]

  16. Polymerized lipid vesicles as colorimetric biosensors for biotechnological applications, R. Jelinek, S. Kolusheva, Adv. Biotech., 2001, 19, 1-10.
    [Abstract]

  17. A new colorimetric assay for studying and rapid screening of membrane penetration enhancers, D. Evrard, E. Touitou, S. Kolusheva, Y. Fishov, R. Jelinek, Pharm. Res., 2001, 18, 943-949.
    [Abstract]

  18. Peptide-membrane interactions studied by a new phospholipid/polydiacetylene colorimetric vesicle assay, S. Kolusheva, T. Shahal, R. Jelinek, Biochemistry, 2000, 39, 15851-15859.
    [Abstract] [Full Paper]