The Blaustein Institute for Desert Research -- Department of Dryland Biotechnologies -- Microalgae Laboratory
|| CLAUDE AFLALO
Born: 1949, Fes, Morocco|
Ph.D.: 1986, Ben-Gurion University|
Senior Lecturer: 1999|
See also the underlying ideas, a glossary of terms, as well as some highlights.
- Cellular bioenergetics and regulation of metabolism through micro-compartmentation.
- Functional consequences of cellular organization; assessment of the role of physical phenomena such as
in cellular energetics.
- macromolecular association
- Signal transduction through extracellular adenine nucleotides.
- Bioenergetics in extremophile microalgae.
- Enzymes kinetics in situ (measurements and mathematical modeling of heterogenous catalytic processes).
- Protein traffic and assembly (firefly luciferase as a probe).
- Molecular biology including the study of structure, function and regulation of metabolic units.
- In silico assessment and modeling of macromolecular recognition.
- Development of experimental approaches using genetically engineered, cloned firefly luciferase as a tool to measure local ATP concentrations in intact cells and isolated organelles (yeast).
- Firefly luciferase mechanism: kinetics of light production.
- Cellular protein traffic using luciferase as a reporter for intracellular localization and assembly.
- Interaction of rat brain hexokinase with mitochondrial porin from various sources.
- Involvement of geometric and hydrophobic surface complementarity in macromolecular association.
- Characterization of an extracellular ATP receptor from frog respiratory epithelium.
- Molecular biology, towards transformation and genetic engineering of H. pluvialis, with emphasis on cloning, transformation and over-expression of heterologous genes, and use of firefly luciferase as a reporter gene;
- Cells fractionation and organelles (chloroplast and mitochondria) isolation, towards algal cellular biochemistry, including analysis of protein and enzymes levels and their coordination in anabolic pathways pertinent to the Unitís interests (carotenoids, photosynthesis, and lipid synthesis);
- Experimental framework for the action and regulation of carotenoids biosynthetic pathway, and its relation with lipid synthesis, including quantitation of metabolic intermediates and fluxes under various physiological conditions
- Katchalski-Katzir, E, Shariv, I, Eisenstein, M, Friezem, AA, Aflalo, C, and Vakser, IA. Molecular surface recognition: determination of geometric fit between proteins and their ligands by correlation techniques. Proc. Natl. Acad. Sci. USA. 89:2195-2199, 1992.
- Aflalo, C, and Segel, LA. Local probes and heterogeneous catalysis: a case study of a mitochondria-luciferase-hexokinase system. J. Theor. Biol. 158:67-108, 1992.
- Vakser, I A, and Aflalo, C. Hydrophobic Docking: A Proposed Enhancement to Molecular Recognition Techniques. Proteins: Structure, Function and Genetics, 20:320-329, 1994.
- Aflalo, C. Localized firefly luciferase probes ATP at the surface of mitochondria.
J. Bioenerg. Biomembr. 29:549-559, 1997.
- Aflalo, C, and Azoulay, H. Binding of rat brain hexokinase to recombinant yeast mitochondria: effect of environmental factors and the source of porin J. Bioenerg. Biomembr.30:245-255, 1998.
- Aflalo, C, Bing, W, Zarka A, & Boussiba, S. Effect of the herbicide glufosinate (BASTA) on astaxanthin accumulation in the green alga Haematococcus pluvialis. Z. Naturforsch.54:49-54, 1999.
- Azoulay-Zohar, H, and Aflalo, C. Binding of rat brain hexokinase to recombinant yeast mitochondria: identification of necessary molecular determinants. J. Bioenerg. Biomembr.31:567-577, 1999.
Key words: Firefly luciferase, organization of metabolism, protein traffic and assembly, cellular enzymology, porin, VDAC, ion transport and bioenergetics in microalgea, astaxanthin production, Haematococcus.
Phone: 08-6596817 / 23