Prof. Jerry Eichler: Publications 

Books

Eichler, J. (editor) (2005) Protein Movement Across Membranes. Landes Biosciences/Eurekah.com, Georgetown, TX and Springer Science+Business Media, NY.

Articles

1. Eichler J., Silman I., Gentry M.K. and Anglister L. (1990) The immunocytochemical localization of phosphatidylinositol-anchored acetylcholinesterase in excitable tissues of Torpedo ocellata. Mol. Brain Res. 8:213-218.

2. Eichler J., Toker L. and Silman I. (1991) The effects of heat shock on acetylcholinesterase in cultured chick muscle. FEBS Lett. 293:16-20.

3. Eichler J., SilmanI. and Anglister L. (1992) G2-acetylcholinesterase is presynaptically localized in Torpedo electric organ. J. Neurocytol., 21:707-716.

4. Eichler J., Anselmet A., Sussman J.L., Massoulié J. and SilmanI. (1994) Differential effects of ‘peripheral’ site ligands on Torpedo and chicken acetylcholinesterase. Mol. Pharmacol., 45:335-340.

5. Chatel J.M., Eichler J., Vallette F.M., Bon S., Massoulié J. and Grassi J. (1994) Two-site immunoradiometric assay of chicken acetylcholinesterase: active and inactive molecular forms in brain and muscle. J. Neurochem., 63:1111-1118.

6. Eichler J., Kreimer D.I., Varon L., Silman I. and Weiner L. (1994) A ‘molten globule’ of Torpedo acetylcholinesterase undergoes thiol-disulfide exchange. J. Biol. Chem., 269:30093 30096.

7. Eichler J. and SilmanI. (1995), The activity of an endoplasmic reticulum-localized pool of acetylcholinesterase is modulated by heat shock. J. Biol. Chem., 270:4466-4472.

8. Eichler J., Tolliday N. Toker L. and Silman I. (1995) Purification and characterization of HSP70 from Torpedo electric organ. Comp. Biochem. Physiol. B, 110:409-415.

9. Douville K., Price A., Eichler J., Economou A. and Wickner W. (1995) SecYEG and SecA are the stoichiometric components of preprotein translocase. J. Biol. Chem., 270:20106-20111.

10. Eichler J., Brunner J. and Wickner W. (1997) The protease-protected 30 kDa domain of SecA is largely inaccessible to the membrane lipid phase. EMBO J., 16:2188-2196.

11. Eichler J. and Wickner W. (1997) Both an N-terminal 65 kDa domain and a C-terminal 30 kDa domain of SecA cycle into the membrane at SecYEG during translocation. Proc. Natl. Acad. Sci. USA, 94:5574-5581.

12. Duong F., Eichler J., Price A., Leonard M.R. and Wickner W. (1997) Biogenesis of the E. coli envelope. Cell, 91:567-573.

13. Anglister L., Eichler J., Szabo M., Haesaert B. and Salpeter M.M. (1998) [125I]-labeled fasciculin 2. A new tool for quantitation of acetylcholinesterase densities at synaptic sites by EM autoradiography. J. Neurosci. Meth., 81:63-71.

14. Eichler J., Rinard K. and Wickner W. (1998) SecA catalysis of preprotein translocation occurs exclusively at SecYEG. J. Biol.Chem., 273:21675-21681.

15. Eichler J. and Wickner W. (1998) The SecA subunit of Escherichia coli preprotein translocase is exposed to the periplasm. J. Bacteriol., 180:5776-5779.

16. Vyazmensky, M., Barak, Z., Chipman D. M. and Eichler, J. (2000) Characterization of acetohydroxyacid synthetase activity in the archaeon Haloferax volcanii.Comp. Biochem. Physiol. B, 125:205-210.

17. Eichler, J. (2000) Archaeal protein translocation: crossing membranes in the third domain of life. Eur. J. Biochem., 267:3402-3412.

18. Eichler, J. (2000) Novel glycoproteins of the halophilic archaeon Haloferax volcanii. Arch. Microbiol., 173:445-448.

19. Eichler, J. and Moll, R., (2001) The signal recognition particle of archaea. Trends in Microbiol., 9:130-136.

20. Eichler, J. (2001) Biotechnological uses of archaeal extremozymes. Biotech. Adv., 19:261 278.

21. Ring, G. and Eichler, J. (2001) Large-scale preparation and characterization of inverted membrane vesicles from the halophilic archaeon Haloferax volcanii. J. Membr. Biol., 183:195-204.

22. Eichler, J. (2001) Post-translational modification unrelated to protein glycosylation follows translocation of the S-layer glycoprotein across the plasma membrane of the haloarchaeon Haloferax volcanii. Eur. J. Biochem. 268:4366-4373.

23. Zwieb, C. and Eichler, J. (2002) Getting on target: The archaeal signal recognition particle. Archaea, 1: 27-34.

24. Eichler, J. (2002) Archaeal signal peptidases from the genus Thermoplasma: structural and mechanistic hybrids of the bacterial and eukaryal enzymes. J. Mol. Evol., 54: 411-415.

25. Konrad, Z. and Eichler, J. (2002) Protein glycosylation in Haloferax volcanii: Partial characterization of a 98 kDa glycoprotein. FEMS Microbiol. Lett., 209: 197-202.

26. Konrad, Z. and Eichler, J. (2002) Lipid modification of proteins in Archaea: attachment of a mevalonic acid-based lipid moiety to the S-layer glycoprotein of Haloferax volcanii follows protein translocation. Biochem. J., 366:959-964.

27. Tozik, I., Huang, Q., Zwieb, C. and Eichler, J. (2002) Reconstitution of the signal recognition particle of the halophilic archaeaon Haloferax volcanii. Nucleic Acids Res., 30, 4166-4175.

28. Irihimovitch, V., Ring, G., Elkayam, T., Konrad, Z. and Eichler, J. (2003) Isolation of fusion proteins containing SecY and SecE, components of the protein translocation complex from the halophilic archaeon Haloferax volcanii. Extremophiles, 7:71-77.

29. Irihimovitch, V. and Eichler, J. (2003) Post-translational secretion of fusion proteins in the halophilic archaeon Haloferax volcanii. J. Biol.Chem., 278: 12881-12887

30. Eichler, J. (2003) Evolution of the prokaryotic protein translocation complex: a comparison of archaeal and bacterial versions of SecDF. Mol. Phylogenet. Evol., 27:504-509.

31. Eichler, J. (2003) Protein Targeting Across Evolution: SRP in the Three Domains of Life (invited article). ASM News, 69:277-281.

32. Bardy, S.L., Eichler, J. and Jarrell, K.F. (2003) Archaeal signal peptides - a comparative survey at the genome level. Protein Sci., 12: 1833-1843.

33. Rozner, S., Kolusheva, S., Cohen, Z., Dowhan, W., Eichler, J. and Jelinek, R. (2003) Detection and analysis of membrane interactions by a biomimetic colorimetric lipid/polydiacetylene assay. Anal. Biochem., 319:96-104

34. Eichler, J. and Irihimovitch, V. (2003). Move it on over: Getting proteins across biological membranes (invited review). BioEssays, 25:1154-1157

35. Eichler, J.  (2003) Facing extremes: Archaeal surface-layer (glyco-)proteins. Microbiology, 149: 3347-3351

36. Zeiri, L., Bronk, B.V., Shabtai, Y., Eichler, J. and Efrima, S. (2004) Surface Enhanced Raman Spectroscopy (SERS) as a tool for mapping of specific biochemical components in bacteria. Appl. Spectrosc., 58:33-40.

37. Ring, G. and Eichler, J. (2004) Extreme secretion: Protein translocation across the archaeal plasma membrane. Journal of Bioenergetics and Biomembranes, 36:35-45.

38. Shmuely, H., Dinitz, L., Dahan, I., Eichler, J., Fischer, D., and Shaanan, B. (2004) Poorly conserved ORFs in the genome of the archaea Halobacterium sp. NRC-1 correspond to expressed proteins. Bioinformatics, 20:1248-1253

39. Ring, G. and Eichler, J. (2004) Membrane binding of ribosomes occurs at SecYE-based sites in the Archaea Haloferax volcanii. Journal of Molecular Biology, 336:997-1010.

40. Eichler, J. and Duong, F. (2004) Break on through to the other side – The Sec translocon. Trends Biochem. Sci., 29:221-223.

41. Lichi, T., Ring, G., and Eichler, J. (2004) Membrane binding of SRP pathway components in the halophilic archaea Haloferax volcanii. Eur. J. Biochem., 271:1382-1390.

42. Ring, G. and Eichler, J. (2004) In the Archaea Haloferax volcanii, Membrane Protein Biogenesis and Protein Synthesis Rates Are Affected by Decreased Ribosomal Binding to the Translocon J. Biol. Chem. 279: 53160-53166

43. Eichler, J. and Adams, M.W.W. (2005) Post-translational  protein modification in Archaea. Microbiol. Mol. Biol. Rev., 69: 393-425. Please download the Erratum as well

44. Fine, A., Irihimovitch, V., Dahan, I., Konrad, Z. and Eichler, J. (2006) Cloning, expression and purification of functional Sec11a and Sec11b, type I signal peptidases of the archaeon Haloferax volcanii. J. Bacteriol., 188, 1911-1919.

45. Dinitz-Bab, E., Shmuely, H., Maupin-Furlow, J., Eichler, J. and Shaanan, B. (2006) Haloferax volcanii PitA: An example of functional interaction between the Pfam chlorite dismutase and antibiotic biosynthesis monooxygenase families? Bioinformatics, 22: 671-675.

46. Abu-Qarn, M. and Eichler J. (2006) Protein N-glycosylation in Archaea: Defining Haloferax volcanii genes involved in S-layer glycoprotein glycosylation. Mol. Microbiol., 61, 511-525.

47. Abu-Qarn, M. and Eichler, J. (2006) An analysis of amino acid sequences surrounding archaeal glycoprotein sequons. Archaea, 2, 73-81.

48. Yurist, S., Dahan, I. and Eichler, J. (2007) SRP19 is a dispensable component of the signal recognition particle in Archaea. J. Bacteriol., 189:276-279.

49. Ring, G., Londei, P. and Eichler, J. (2007) Protein biogenesis in Archaea: Addressing translation initiation using an in vitro protein synthesis system for Haloferax volcanii. FEMS Microbiol. Lett., 270, 34-41.

50. Abu-Qarn, M., Yurist-Doutsch, S., Giordano, A., Trauner A., Morris H.R., Hitchen P., Medalia, O., Dell, A. and Eichler, J. (2007) Haloferax volcanii AglB and AglD are involved in N-glycosylation of the S-layer glycoprotein and proper assembly of the surface layer. J. Mol. Biol., 374, 1224-1236.

51. Fink-Lavi, E. and Eichler, J. (2008) Identification of residues essential for the catalytic activity of Sec11b, one of the two type I signal peptidases of Haloferax volcanii. FEMS Microbiol. Lett., 278, 257-260.